Hotspot

psbI-psbK

LSC · 54284-54743 · 460 bp

Back to Pueraria

Pi 0.0105

MarkerSeek score 55.10

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 13
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	TTACGTCCCGGATCGTTAGA	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	58.0	58.1	585-587 bp	1.0	81.3
psbI-psbK_p2	TTACGTCCCGGATCGTTAGA	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	58.0	57.9	580-582 bp	1.0	81.3
psbI-psbK_p3	ACGTCCCGGATCGTTAGATA	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	57.7	58.1	583-585 bp	1.0	79.5
psbI-psbK_p4	TACGTCCCGGATCGTTAGAT	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	57.7	58.1	584-586 bp	1.0	79.5
psbI-psbK_p5	ACGTCCCGGATCGTTAGATA	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	57.7	57.9	578-580 bp	1.0	79.5