Hotspot

atpI-atpH

LSC · 47330-48432 · 1103 bp

Back to Pueraria

Pi 0.00762

MarkerSeek score 39.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 20
pi: 0.00762
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCTGCTGTGGAAGTAGGTCA	GCAGCAATAACAGAAGCAGC	50.0	50.0	58.0	58.1	1827-1837 bp	1.0	80.6
atpI-atpH_p2	TCTGCTGTGGAAGTAGGTCA	CCTTCCCCTGTGTTCTTGTC	50.0	55.0	58.0	58.1	2161-2171 bp	1.0	80.4
atpI-atpH_p3	TCTGCTGTGGAAGTAGGTCA	CTTCCCCTGTGTTCTTGTCC	50.0	55.0	58.0	58.1	2160-2170 bp	1.0	80.4
atpI-atpH_p4	TCTGCTGTGGAAGTAGGTCA	GTCCTTCCCCTGTGTTCTTG	50.0	55.0	58.0	58.1	2163-2173 bp	1.0	80.4
atpI-atpH_p5	TCTGCTGTGGAAGTAGGTCA	AAGCGCTAATGCTACAACCA	50.0	45.0	58.0	57.9	2011-2021 bp	1.0	80.4