Hotspot

rps2-atpI

LSC · 46346-46585 · 240 bp

Back to Pueraria

Pi 0.00923

MarkerSeek score 48.10

Primer available yes

Label rps2-atpI

Feature Metadata

label_name: rps2-atpI
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 6
pi: 0.00923
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-atpI_p1	TGCAATTTGTGAGGGTCGTT	TGACCTACTTCCACAGCAGA	45.0	50.0	58.3	58.0	354-359 bp	1.0	78.8
rps2-atpI_p2	TTGCAATTTGTGAGGGTCGT	TGACCTACTTCCACAGCAGA	45.0	50.0	58.3	58.0	355-360 bp	1.0	78.8
rps2-atpI_p3	GCAATTTGTGAGGGTCGTTC	TGACCTACTTCCACAGCAGA	50.0	50.0	57.7	58.0	353-358 bp	1.0	78.6
rps2-atpI_p4	TTGTGAGGGTCGTTCTAGCT	TGACCTACTTCCACAGCAGA	50.0	50.0	58.4	58.0	348-353 bp	1.0	78.3
rps2-atpI_p5	TTTGTGAGGGTCGTTCTAGC	TGACCTACTTCCACAGCAGA	50.0	50.0	57.5	58.0	349-354 bp	1.0	77.7