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psbM-petN

LSC · 31975-32709 · 735 bp

Back to Pueraria

Pi 0.00511

MarkerSeek score 52.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 11
pi: 0.00511
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	38.1	45.0	55.2	58.0	872-890 bp	1.0	53.5
psbM-petN_p2	TGCTACTGCACTGTTTATTCT	AGTAAGTCTTGCTTGGGCTG	38.1	50.0	55.2	57.8	878-896 bp	1.0	52.0
psbM-petN_p3	TGCTACTGCACTGTTTATTCTAGT	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	58.0	58.0	872-890 bp	1.0	51.2
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	CCCTCGTAGTATGGGGAAGA	38.1	55.0	55.2	57.6	828-846 bp	1.0	50.7
psbM-petN_p5	ACTGCACTGTTTATTCTAGTTCCT	TCTTGCTTGGGCTGCTTTAA	37.5	45.0	57.9	58.0	868-886 bp	1.0	50.6