Hotspot

rps14-trnP-GGG

LSC · 24238-24427 · 190 bp

Back to Pueraria

Pi 0.019

MarkerSeek score 52.00

Primer available yes

Label rps14-trnP-GGG

Feature Metadata

label_name: rps14-trnP-GGG
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 8
pi: 0.019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-trnP-GGG_p1	CATGCATGTTTATTGCCCGG	AAGGTTATGAGCCTTGCGAG	50.0	50.0	58.1	58.0	263-275 bp	1.0	84.6
rps14-trnP-GGG_p2	CATGCATGTTTATTGCCCGG	TTTGAACCCGTGACCTCAAG	50.0	50.0	58.1	58.0	280-292 bp	1.0	84.2
rps14-trnP-GGG_p3	CATGCATGTTTATTGCCCGG	GATTTGAACCCGTGACCTCA	50.0	50.0	58.1	57.8	282-294 bp	1.0	83.0
rps14-trnP-GGG_p4	GACCGCGAGCTAATTATCGA	AAGGTTATGAGCCTTGCGAG	50.0	50.0	57.7	58.0	322-334 bp	1.0	82.1
rps14-trnP-GGG_p5	ACCGCGAGCTAATTATCGAG	AAGGTTATGAGCCTTGCGAG	50.0	50.0	57.7	58.0	321-333 bp	1.0	82.0