Hotspot

rps16

LSC · 4522-5606 · 1085 bp

Back to Puccinellia

Pi 0.000925

MarkerSeek score 59.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1085
variable_sites: 1
pi: 0.000925
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GATTGGCGCGACATAAATCC	ACCACTTTGCAATGAAAAGAGG	50.0	40.9	57.7	57.7	1232-1235 bp	1.0	59.2
rps16_p2	GGATTGGCGCGACATAAATC	ACCACTTTGCAATGAAAAGAGG	50.0	40.9	57.7	57.7	1233-1236 bp	1.0	59.2
rps16_p3	ATTGGCGCGACATAAATCCA	ACCACTTTGCAATGAAAAGAGG	45.0	40.9	58.3	57.7	1231-1234 bp	1.0	59.1
rps16_p4	TGGATTGGCGCGACATAAAT	ACCACTTTGCAATGAAAAGAGG	45.0	40.9	58.3	57.7	1234-1237 bp	1.0	59.1
rps16_p5	TGGCGCGACATAAATCCAAA	ACCACTTTGCAATGAAAAGAGG	45.0	40.9	58.5	57.7	1229-1232 bp	1.0	57.3