Hotspot

ndhH

SSC · 113192-114373 · 1182 bp

Back to Puccinellia

Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AAAGGAGGCTCAAAATGGGT	TGCATTTTGATGAGCCTCGT	45.0	45.0	57.3	58.2	1371 bp	1.0	73.1
ndhH_p2	TCCCAAAAGGAGGCTCAAAA	TGCATTTTGATGAGCCTCGT	45.0	45.0	57.2	58.2	1376 bp	1.0	72.6
ndhH_p3	AAAAGGAGGCTCAAAATGGGT	TGCATTTTGATGAGCCTCGT	42.9	45.0	58.0	58.2	1372 bp	1.0	70.5
ndhH_p4	GGAGGCTCAAAATGGGTAGA	TGCATTTTGATGAGCCTCGT	50.0	45.0	56.9	58.2	1368 bp	1.0	69.9
ndhH_p5	AGGAGGCTCAAAATGGGTAG	TGCATTTTGATGAGCCTCGT	50.0	45.0	56.9	58.2	1369 bp	1.0	69.8