Hotspot

ndhF

SSC · 101579-103798 · 2220 bp

Back to Puccinellia

Pi 0.0018

MarkerSeek score 43.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACACCCATCAATGCATGGT	ACGAAACAAGAGAGCTGCTT	45.0	45.0	58.1	57.8	2786-2791 bp	1.0	77.9
ndhF_p2	ACCCATCAATGCATGGTGTT	ACGAAACAAGAGAGCTGCTT	45.0	45.0	58.1	57.8	2783-2788 bp	1.0	77.9
ndhF_p3	TGCATTTTGATGAGCCTCGT	ACGAAACAAGAGAGCTGCTT	45.0	45.0	58.2	57.8	2917-2922 bp	1.0	76.9
ndhF_p4	GCATGGTGTTCTTCGACTGA	ACGAAACAAGAGAGCTGCTT	50.0	45.0	58.2	57.8	2773-2778 bp	1.0	76.7
ndhF_p5	GAAGAGGGAGAGGACAGGAT	GGTATGGAAGCCTATCCCCT	55.0	55.0	57.5	58.0	2880-2887 bp	1.0	76.2