Pi
0.00403
MarkerSeek score
46.30
Primer available
yes
Label
atpI
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| atpI_p1 | ATCAGCTGCAATAAGGGCTC | TTCTGGCTGTCTCGCAATAC | 50.0 | 50.0 | 58.0 | 58.0 | 2161 bp | 1.0 | 79.9 |
| atpI_p2 | CTCTTAGGGAATGCGCCATT | TTCTGGCTGTCTCGCAATAC | 50.0 | 50.0 | 58.0 | 58.0 | 1889 bp | 1.0 | 79.9 |
| atpI_p3 | ATCAGCTGCAATAAGGGCTC | AACCCGCACAAAAGATGACT | 50.0 | 45.0 | 58.0 | 58.0 | 1712 bp | 1.0 | 79.9 |
| atpI_p4 | CTCTTAGGGAATGCGCCATT | AACCCGCACAAAAGATGACT | 50.0 | 45.0 | 58.0 | 58.0 | 1440 bp | 1.0 | 79.9 |
| atpI_p5 | AGATATCTCGGCGGGATCAA | TTCTGGCTGTCTCGCAATAC | 50.0 | 50.0 | 58.1 | 58.0 | 1968 bp | 1.0 | 79.5 |