Hotspot

trnC-GCA-rpoB

LSC · 17943-19080 · 1138 bp

Back to Puccinellia

Pi 0.00351

MarkerSeek score 60.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 4
pi: 0.00351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1207 bp	1.0	79.4
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1214 bp	1.0	78.3
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TGTCAAGTTCTTCTGCCAGG	50.0	50.0	58.1	57.7	1290 bp	1.0	77.6
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1222 bp	1.0	77.1
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1221 bp	1.0	77.1