Hotspot

rpoC1

LSC · 21166-23965 · 2800 bp

Back to Pterygiella

Pi 0.00143

MarkerSeek score 56.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2800
variable_sites: 4
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GGGACTGTGGGACTAGTTGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.4	58.0	2988-2994 bp	1.0	76.9
rpoC1_p2	GGGACTGTGGGACTAGTTGA	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.4	57.5	2986-2992 bp	1.0	73.6
rpoC1_p3	CGTAGAAATTCCGGGACTGT	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.3	58.0	3000 bp	0.5	54.3
rpoC1_p4	TAGAAATTCCGGGACTGTGG	TTGCTCGTTCGAGAACTACG	50.0	50.0	56.9	58.0	2998 bp	0.5	51.1
rpoC1_p5	CGTAGAAATTCCGGGACTGT	GCTCGTTCGAGAACTACGAT	50.0	50.0	57.3	57.5	2998 bp	0.5	51.0