Hotspot

ndhG

SSC · 118961-119491 · 531 bp

Back to Pterygiella

Pi 0.00188

MarkerSeek score 45.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGAATTACTCTTTTTAAAGGTTGC	GTCCTCGTCAGGTCAACAAA	29.2	50.0	54.3	57.8	611 bp	1.0	40.1
ndhG_p2	TGAATTACTCTTTTTAAAGGTTGC	TTTGTCCTCGTCAGGTCAAC	29.2	50.0	54.3	57.8	614 bp	1.0	40.1
ndhG_p3	TGAATTACTCTTTTTAAAGGTTGC	CCTCGTCAGGTCAACAAAGG	29.2	55.0	54.3	58.5	609 bp	1.0	40.1
ndhG_p4	TTGAATTACTCTTTTTAAAGGTTGC	GTCCTCGTCAGGTCAACAAA	28.0	50.0	55.0	57.8	612 bp	1.0	40.1
ndhG_p5	TTGAATTACTCTTTTTAAAGGTTGC	TTTGTCCTCGTCAGGTCAAC	28.0	50.0	55.0	57.8	615 bp	1.0	40.1