Hotspot

ndhD

SSC · 116341-117945 · 1605 bp

Back to Pterygiella

Pi 0.00125

MarkerSeek score 53.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1605
variable_sites: 2
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGGGTTCTCGCTAGTTCAAA	GTGAATCTGCCTGTCCAACA	50.0	50.0	57.9	58.1	1810 bp	1.0	78.7
ndhD_p2	CGGGTTCTCGCTAGTTCAAA	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.9	58.1	1811 bp	1.0	78.7
ndhD_p3	CGGGTTCTCGCTAGTTCAAA	TGAATCTGCCTGTCCAACAG	50.0	50.0	57.9	57.8	1809 bp	1.0	78.0
ndhD_p4	CGGGTTCTCGCTAGTTCAAA	GATGTGAATCTGCCTGTCCA	50.0	50.0	57.9	57.6	1813 bp	1.0	76.2
ndhD_p5	CGGGTTCTCGCTAGTTCAAA	AGATGTGAATCTGCCTGTCC	50.0	50.0	57.9	57.3	1814 bp	1.0	73.8