Hotspot

psbE-petL

LSC · 66267-67140 · 874 bp

Back to Pterygiella

Pi 0.00343

MarkerSeek score 60.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 3
pi: 0.00343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	989-1036 bp	1.0	71.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	984-1031 bp	1.0	71.1
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	982-1029 bp	1.0	69.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	989-1036 bp	1.0	69.1
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	990-1037 bp	1.0	69.1