Hotspot

ycf4-cemA

LSC · 61569-62524 · 956 bp

Back to Pterygiella

Pi 0.00748

MarkerSeek score 51.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 7
pi: 0.00748
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCAAGGGATCGTGATGTCTT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1383-1402 bp	1.0	80.8
ycf4-cemA_p2	CGTTGGGGATTTCCTGGAAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1250-1269 bp	1.0	80.5
ycf4-cemA_p3	TTTCGTTGGGGATTTCCTGG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1253-1272 bp	1.0	80.5
ycf4-cemA_p4	TGGTGCACAATTTCCTGGAA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	1325-1344 bp	1.0	80.4
ycf4-cemA_p5	GCAAGGGATCGTGATGTCTT	GTGGGGTGAATGAAATCCGA	50.0	50.0	58.0	57.9	1814-1833 bp	1.0	80.0