Hotspot

ndhC-trnV-UAC

LSC · 51134-52291 · 1158 bp

Back to Pterygiella

Pi 0.00259
MarkerSeek score 60.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1158
variable_sites
3
pi
0.00259
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.9 57.9 1278-1284 bp 1.0 78.9
ndhC-trnV-UAC_p2 GGGGCTAAAATCCCGGAAAT CGGTTCGAATCCGTATAGCC 50.0 55.0 57.9 58.3 1279-1285 bp 1.0 77.1
ndhC-trnV-UAC_p3 TTCTCCGGCCCTTTCCTAAT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.4 57.9 1299-1305 bp 1.0 76.3
ndhC-trnV-UAC_p4 CCCTTTCCTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 55.0 55.0 58.6 57.9 1291-1297 bp 1.0 75.0
ndhC-trnV-UAC_p5 TTTCTCCGGCCCTTTCCTAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.6 57.9 1300-1306 bp 1.0 74.5