Hotspot

trnK-UUU-rps16

LSC · 4147-5043 · 897 bp

Back to Pterygiella

Pi 0.00447

MarkerSeek score 61.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 4
pi: 0.00447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	1020-1023 bp	1.0	65.9
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GTGCTCAACCCACAGGAAC	50.0	57.9	58.2	58.7	1019-1022 bp	1.0	65.9
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	1105-1108 bp	1.0	65.3
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	GTGCTCAACCCACAGGAAC	50.0	57.9	57.8	58.7	1104-1107 bp	1.0	65.2
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	GTGCTCAACCCACAGGAAC	50.0	57.9	58.3	58.7	1034-1037 bp	1.0	65.1