Hotspot

psbT-psbN

LSC · 78148-78218 · 71 bp

Back to Pteroxygonum

Pi 0.0704

MarkerSeek score 80.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 5
pi: 0.0704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.6	58.1	134 bp	1.0	79.4
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.6	58.2	135 bp	1.0	78.8
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.5	58.1	132 bp	1.0	78.6
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	ACCCTAGTCGCCATCTTTCT	50.0	50.0	57.6	58.1	225 bp	1.0	77.8
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	ACCCTAGTCGCCATCTTTCT	50.0	50.0	57.5	58.1	223 bp	1.0	77.0