Hotspot

rpl20-rps12

LSC · 72685-73463 · 779 bp

Back to Pteroxygonum

Pi 0.0342

MarkerSeek score 80.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 26
pi: 0.0342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCCCTCGAAAGCTTGATACA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.3	57.9	866-885 bp	1.0	77.6
rpl20-rps12_p2	GCCCCTCGAAAGCTTGATAC	CGCCGAGGAACATGTACTAG	55.0	55.0	58.7	57.9	867-886 bp	1.0	77.5
rpl20-rps12_p3	TCCTCGTCTAACTCTGGTCA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.1	57.9	808-827 bp	1.0	76.4
rpl20-rps12_p4	ATAGGTTTTGAATGCGCCCC	CGCCGAGGAACATGTACTAG	50.0	55.0	58.9	57.9	882-901 bp	1.0	76.0
rpl20-rps12_p5	AATAGGTTTTGAATGCGCCC	CGCCGAGGAACATGTACTAG	45.0	55.0	57.0	57.9	883-902 bp	1.0	75.4