Hotspot

ycf4-cemA

LSC · 63734-64480 · 747 bp

Back to Pteroxygonum

Pi 0.0385

MarkerSeek score 73.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 28
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	AGAAAGCTCCACCAGGGTAA	45.0	50.0	57.8	58.3	876-976 bp	1.0	80.8
ycf4-cemA_p2	TATTTCTTGCGCGTACCGAT	AGAAAGCTCCACCAGGGTAA	45.0	50.0	57.8	58.3	854-954 bp	1.0	80.4
ycf4-cemA_p3	GGAACAAAAAGCTGCCGAAT	AGAAAGCTCCACCAGGGTAA	45.0	50.0	57.6	58.3	879-979 bp	1.0	78.5
ycf4-cemA_p4	CTATTTCTTGCGCGTACCGA	AGAAAGCTCCACCAGGGTAA	50.0	50.0	58.4	58.3	855-955 bp	1.0	78.5
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	GCTCCACCAGGGTAAAAAGA	45.0	50.0	57.8	57.4	871-971 bp	1.0	78.2