Hotspot

ndhC-trnV-UAC

LSC · 53791-54919 · 1129 bp

Back to Pteroxygonum

Pi 0.0546
MarkerSeek score 78.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1129
variable_sites
54
pi
0.0546
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGAAAGTAGAAATGCCA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.8 57.8 1159-1249 bp 1.0 76.7
ndhC-trnV-UAC_p2 TCCGGAAAGTAGAAATGCCAA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.6 57.8 1159-1249 bp 1.0 74.6
ndhC-trnV-UAC_p3 TTCCGGAAAGTAGAAATGCCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.6 57.8 1160-1250 bp 1.0 74.6
ndhC-trnV-UAC_p4 TCCGGAAAGTAGAAATGCCA GAAGGTCTACGGTTCGAGTC 45.0 55.0 56.8 57.5 1158-1248 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCCGGAAAGTAGAAATGCCA GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.8 58.7 1147-1237 bp 1.0 72.9