Hotspot

trnS-UGA-psbZ

LSC · 37906-38234 · 329 bp

Back to Pteroxygonum

Pi 0.0396

MarkerSeek score 66.00

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 13
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ACTTGACCAACCATCAGGAG	55.0	50.0	58.0	57.1	455-460 bp	1.0	75.3
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.0	57.0	383-388 bp	1.0	74.8
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	ACTTGACCAACCATCAGGAG	50.0	50.0	57.9	57.1	475-480 bp	1.0	74.4
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	TGCAAAAACAGCCAATTGGA	50.0	40.0	57.9	57.0	403-408 bp	1.0	73.8
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GGAGATGCAAATACGACAGGT	55.0	47.6	58.0	58.1	439-444 bp	1.0	73.6