Hotspot

trnC-GCA-petN

LSC · 28833-29669 · 837 bp

Back to Pteroxygonum

Pi 0.0398

MarkerSeek score 74.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 32
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCGGTTCAAATCTGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	893-930 bp	1.0	83.7
trnC-GCA-petN_p2	TTTCCCCGGTTCAAATCTGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	892-929 bp	1.0	77.8
trnC-GCA-petN_p3	CCGGTTCAAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	888-925 bp	1.0	77.8
trnC-GCA-petN_p4	TTTCCCCGGTTCAAATCTGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	901-938 bp	1.0	77.7
trnC-GCA-petN_p5	TTTCCCCGGTTCAAATCTGG	GACTACCATTAAAGCAGCCCA	50.0	47.6	58.1	58.0	908-945 bp	1.0	76.9