Hotspot

rpoB-trnC-GCA

LSC · 27450-28760 · 1311 bp

Back to Pteroxygonum

Pi 0.0369

MarkerSeek score 74.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1311
variable_sites: 47
pi: 0.0369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCCCAGGTATTGTCGACATT	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.0	58.1	1380-1417 bp	1.0	83.9
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.0	58.1	1433-1470 bp	1.0	83.8
rpoB-trnC-GCA_p3	GCCCAGGTATTGTCGACATT	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.0	58.2	1360-1397 bp	1.0	83.2
rpoB-trnC-GCA_p4	CGTCAAGCCCTGATCAATGA	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.0	58.2	1413-1450 bp	1.0	83.1
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.4	58.1	1443-1480 bp	1.0	80.8