Hotspot

trnS-GCU-trnG-UCC

LSC · 8954-9860 · 907 bp

Back to Pteroxygonum

Pi 0.0755

MarkerSeek score 86.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 66
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCGTCCACTCAGCCATCTAT	CGGATTAGGAATCGAAGGATCC	50.0	50.0	57.9	58.1	1000 bp	0.5	42.2
trnS-GCU-trnG-UCC_p2	TCGTCCACTCAGCCATCTAT	GGATTAGGAATCGAAGGATCCC	50.0	50.0	57.9	57.8	999 bp	0.5	41.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	CGGATTAGGAATCGAAGGATCC	50.0	50.0	58.2	58.1	1067 bp	0.5	41.6
trnS-GCU-trnG-UCC_p4	TTCGTCCACTCAGCCATCTA	CGGATTAGGAATCGAAGGATCC	50.0	50.0	58.2	58.1	1001 bp	0.5	41.6
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	GGATTAGGAATCGAAGGATCCC	50.0	50.0	58.2	57.8	1066 bp	0.5	41.1