Hotspot

psbI-trnS-GCU

LSC · 8750-8865 · 116 bp

Back to Pteroxygonum

Pi 0.105

MarkerSeek score 82.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 12
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	GTCATTCGTACCGAGGGTTC	50.0	55.0	57.1	58.1	177 bp	1.0	78.2
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATAGATGGCTGAGTGGACGA	50.0	50.0	57.1	57.9	226 bp	1.0	76.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	TAGATGGCTGAGTGGACGAA	50.0	50.0	57.1	58.2	225 bp	1.0	76.3
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	AGGGATAGATGGCTGAGTGG	50.0	55.0	57.1	58.3	230 bp	1.0	75.2
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GGGATAGATGGCTGAGTGGA	50.0	55.0	57.1	58.3	229 bp	1.0	75.2