Hotspot

rps16-trnQ-UUG

LSC · 6254-7644 · 1391 bp

Back to Pteroxygonum

Pi 0.0627
MarkerSeek score 76.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1391
variable_sites
72
pi
0.0627
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGGCAGCAACATACCCTTTT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.0 57.9 2156-2367 bp 1.0 86.7
rps16-trnQ-UUG_p2 TGGAAACTTGGTGGGATTGG CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 2308 bp 0.5 59.5
rps16-trnQ-UUG_p3 TGGATTGGAAACTTGGTGGG CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 2313 bp 0.5 59.5
rps16-trnQ-UUG_p4 TGGAAACTTGGTGGGATTGG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1749 bp 0.5 59.3
rps16-trnQ-UUG_p5 TGGATTGGAAACTTGGTGGG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1754 bp 0.5 59.3