Hotspot

trnH-GUG-psbA

LSC · 77-417 · 341 bp

Back to Pteroxygonum

Pi 0.123

MarkerSeek score 78.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 38
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	457-465 bp	1.0	89.6
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CTGTTGCGTATTCAGCTCCT	55.0	50.0	58.1	58.0	994-1002 bp	1.0	84.9
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTGTTGCGTATTCAGCTCCT	50.0	50.0	57.9	58.0	1008-1016 bp	1.0	84.8
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CTGTTGCGTATTCAGCTCCT	50.0	50.0	57.9	58.0	1007-1015 bp	1.0	84.8
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CTGTTGCGTATTCAGCTCCT	50.0	50.0	57.9	58.0	1009-1017 bp	1.0	84.8