Hotspot

rps16

LSC · 5203-6322 · 1120 bp

Back to Pterostyrax

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1120
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATCATCACTCCCTCAC	AATTCCATACCGGCTCCAAA	50.0	45.0	57.0	57.2	1191 bp	1.0	65.7
rps16_p2	ACGAATCATCACTCCCTCAC	TTCCATACCGGCTCCAAATT	50.0	45.0	57.0	57.2	1189 bp	1.0	65.7
rps16_p3	ACGAATCATCACTCCCTCAC	ATTCCATACCGGCTCCAAAT	50.0	45.0	57.0	56.9	1190 bp	1.0	63.8
rps16_p4	CGAATCATCACTCCCTCACT	AATTCCATACCGGCTCCAAA	50.0	45.0	56.7	57.2	1190 bp	1.0	63.3
rps16_p5	CGAATCATCACTCCCTCACT	TTCCATACCGGCTCCAAATT	50.0	45.0	56.7	57.2	1188 bp	1.0	63.3