Hotspot

atpF

LSC · 12881-14157 · 1277 bp

Back to Pterostyrax

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1277
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AAAAAGGGGCGAAGTGATAA	50.0	40.0	57.7	55.2	1426-1427 bp	1.0	55.3
atpF_p2	GGCTCGAATGGTTACCATGA	AAAAGGGGCGAAGTGATAAA	50.0	40.0	57.7	55.2	1425-1426 bp	1.0	55.3
atpF_p3	GGCTCGAATGGTTACCATGA	AAAGGGGCGAAGTGATAAAA	50.0	40.0	57.7	55.2	1424-1425 bp	1.0	55.3
atpF_p4	GGCTCGAATGGTTACCATGA	AAGGGGCGAAGTGATAAAAA	50.0	40.0	57.7	55.2	1423-1424 bp	1.0	55.3
atpF_p5	GAATTTCGTCGGCTCGAATG	AAGGGGCGAAGTGATAAAAA	50.0	40.0	57.6	55.2	1433-1434 bp	1.0	55.1