Hotspot

trnS-GCU-trnG-GCC

LSC · 9415-10104 · 690 bp

Back to Pterostyrax

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
690
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 AGGAAACGGAAAGAGAGGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 818 bp 1.0 69.9
trnS-GCU-trnG-GCC_p2 AGGAAACGGAAAGAGAGGGA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 818 bp 1.0 69.8
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCACT 45.0 42.9 58.0 58.2 764 bp 1.0 69.8
trnS-GCU-trnG-GCC_p4 ATTAGCAATCCGCCGCTTTA ACGAATCGCACTTTTACCAC 45.0 45.0 58.0 56.8 764 bp 1.0 69.7
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 813 bp 1.0 68.9