primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	90	480	517-517	1.000000	76.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	58.2	58.3	0.0	0.0	0.496882	90	480	518-518	1.000000	76.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	90	480	518-518	1.000000	75.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.557166	90	480	519-519	1.000000	75.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.7	58.3	0.0	0.0	0.668369	90	480	510-510	1.000000	74.7
psbA_p1	psbA	1	AAAGGAGCAATAACGCCCTC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.267306	481	1542	1206-1206	1.000000	77.9
psbA_p2	psbA	2	AGCAATAACGCCCTCTTCTC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	481	1542	1201-1201	1.000000	77.3
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTCT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	481	1542	1202-1202	1.000000	77.3
psbA_p4	psbA	4	GCAATAACGCCCTCTTCTCT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	481	1542	1200-1200	1.000000	77.3
psbA_p5	psbA	5	GGAGCAATAACGCCCTCTTC	AAATTCGTGTGCTTGGGAGT	20	20	55.0	45.0	58.7	58.0	0.0	0.0	0.718262	481	1542	1203-1203	1.000000	74.3
matK_p1	matK	1	ACGCGGTCTCATAACAAATCA	TGTTTTGACCGTATCGCACT	21	20	42.9	45.0	58.0	57.8	12.5	0.0	1.190472	2066	3589	1621-1621	1.000000	70.5
matK_p2	matK	2	ACGCGGTCTCATAACAAATCA	CCTTGTTTTGACCGTATCGC	21	20	42.9	50.0	58.0	57.5	12.5	0.0	1.553324	2066	3589	1624-1624	1.000000	67.6
matK_p3	matK	3	CGCGGTCTCATAACAAATCA	TGTTTTGACCGTATCGCACT	20	20	45.0	45.0	56.3	57.8	0.0	0.0	1.905180	2066	3589	1620-1620	1.000000	64.8
matK_p4	matK	4	ACGCGGTCTCATAACAAATC	TGTTTTGACCGTATCGCACT	20	20	45.0	45.0	56.3	57.8	12.5	0.0	1.907662	2066	3589	1621-1621	1.000000	64.7
matK_p5	matK	5	ACGCGGTCTCATAACAAATCA	TTGTTTTGACCGTATCGCAC	21	20	42.9	45.0	58.0	57.0	12.5	0.0	1.986278	2066	3589	1622-1622	1.000000	64.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	CGAAGCCGTATTGCTGAGAT	CGATTCGGGTTGCTAACTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.146491	3590	4302	811-811	1.000000	78.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	CGAAGCCGTATTGCTGAGAT	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.1	58.2	0.0	17.1	0.310328	3590	4302	792-792	1.000000	77.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	CGAAGCCGTATTGCTGAGAT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.1	58.2	0.0	17.1	0.310328	3590	4302	791-791	1.000000	77.5
matK-trnK-UUU_p4	matK-trnK-UUU	4	CGAAGCCGTATTGCTGAGAT	TCGATTCGGGTTGCTAACTC	20	20	50.0	50.0	58.1	57.7	0.0	6.2	0.430869	3590	4302	812-812	1.000000	76.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	CGAAGCCGTATTGCTGAGAT	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.602666	3590	4302	805-805	1.000000	75.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTTGCTTCATCCA	AAGGGCGGAGGTTTTTAAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.123446	4340	5202	997-997	1.000000	79.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACGAATCCCTTGCTTCATCC	AAGGGCGGAGGTTTTTAAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.124882	4340	5202	998-998	1.000000	79.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	ATGGACGAATCCCTTGCTTC	AAGGGCGGAGGTTTTTAAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.124882	4340	5202	1002-1002	1.000000	79.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GGACGAATCCCTTGCTTCAT	AAGGGCGGAGGTTTTTAAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.124882	4340	5202	1000-1000	1.000000	79.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGAATCCCTTGCTTCATCCA	GGGCGGAGGTTTTTAAGGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.128908	4340	5202	995-995	1.000000	79.0
rps16_p1	rps16	1	ACGAATCATCACTCCCTCAC	AATTCCATACCGGCTCCAAA	20	20	50.0	45.0	57.0	57.2	0.0	0.0	1.788379	5203	6322	1191-1191	1.000000	65.7
rps16_p2	rps16	2	ACGAATCATCACTCCCTCAC	TTCCATACCGGCTCCAAATT	20	20	50.0	45.0	57.0	57.2	0.0	0.0	1.788379	5203	6322	1189-1189	1.000000	65.7
rps16_p3	rps16	3	ACGAATCATCACTCCCTCAC	ATTCCATACCGGCTCCAAAT	20	20	50.0	45.0	57.0	56.9	0.0	0.0	2.020492	5203	6322	1190-1190	1.000000	63.8
rps16_p4	rps16	4	CGAATCATCACTCCCTCACT	AATTCCATACCGGCTCCAAA	20	20	50.0	45.0	56.7	57.2	0.0	0.0	2.084989	5203	6322	1190-1190	1.000000	63.3
rps16_p5	rps16	5	CGAATCATCACTCCCTCACT	TTCCATACCGGCTCCAAATT	20	20	50.0	45.0	56.7	57.2	0.0	0.0	2.084989	5203	6322	1188-1188	1.000000	63.3
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.262890	9415	10104	818-818	1.000000	69.9
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AGGAAACGGAAAGAGAGGGA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.269916	9415	10104	818-818	1.000000	69.8
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCACT	20	21	45.0	42.9	58.0	58.2	0.0	1.2	1.275461	9415	10104	764-764	1.000000	69.8
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATTAGCAATCCGCCGCTTTA	ACGAATCGCACTTTTACCAC	20	20	45.0	45.0	58.0	56.8	0.0	1.2	1.282487	9415	10104	764-764	1.000000	69.7
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.2	58.2	0.0	1.2	1.388603	9415	10104	813-813	1.000000	68.9
trnG-GCC_p1	trnG-GCC	1	GGCTAATTCCCCTGTTCGAC	AATATAGAGGGTATATAGGAATGAA	20	25	55.0	28.0	58.3	52.1	0.0	0.0	11.243265	10105	10872	934-934	1.000000	40.0
trnG-GCC_p2	trnG-GCC	2	ATGGCTAATTCCCCTGTTCG	AATATAGAGGGTATATAGGAATGAA	20	25	50.0	28.0	57.7	52.1	0.0	0.0	11.256549	10105	10872	936-936	1.000000	40.0
trnG-GCC_p3	trnG-GCC	3	GCTAATTCCCCTGTTCGACA	AATATAGAGGGTATATAGGAATGAA	20	25	50.0	28.0	57.6	52.1	0.0	0.0	11.304728	10105	10872	933-933	1.000000	40.0
trnG-GCC_p4	trnG-GCC	4	TGGCTAATTCCCCTGTTCGA	AATATAGAGGGTATATAGGAATGAA	20	25	50.0	28.0	58.7	52.1	0.0	0.0	11.626719	10105	10872	935-935	1.000000	40.0
trnG-GCC_p5	trnG-GCC	5	GGCTAATTCCCCTGTTCGAC	AGAGGGTATATAGGAATGAATATATT	20	26	55.0	26.9	58.3	52.7	0.0	0.0	11.636535	10105	10872	929-929	1.000000	40.0
atpA_p1	atpA	1	AGAGAGGCTTCGTTACTCCT	TGGTATGTTTGGAGCGATGA	20	20	50.0	45.0	57.5	56.9	0.0	0.0	1.587820	11293	12816	1677-1678	1.000000	67.3
atpA_p2	atpA	2	CAGAGAGGCTTCGTTACTCC	TGGTATGTTTGGAGCGATGA	20	20	55.0	45.0	57.4	56.9	0.0	0.0	1.640265	11293	12816	1678-1679	1.000000	66.9
atpA_p3	atpA	3	AGAGAGGCTTCGTTACTCCT	TGGTATGTTTGGAGCGATGAA	20	21	50.0	42.9	57.5	57.7	0.0	0.0	1.860375	11293	12816	1677-1678	1.000000	65.2
atpA_p4	atpA	4	AGAGAGGCTTCGTTACTCCT	TTGGTATGTTTGGAGCGATGA	20	21	50.0	42.9	57.5	57.7	0.0	0.0	1.860375	11293	12816	1678-1679	1.000000	65.2
atpA_p5	atpA	5	CAGAGAGGCTTCGTTACTCC	TGGTATGTTTGGAGCGATGAA	20	21	55.0	42.9	57.4	57.7	0.0	0.0	1.912819	11293	12816	1678-1679	1.000000	64.7
atpA-atpF_p1	atpA-atpF	1	GAATTTCGTCGGCTCGAATG	TGGTATGTTTGGAGCGATGA	20	20	50.0	45.0	57.6	56.9	13.2	0.0	1.423080	12817	12880	128-129	1.000000	69.2
atpA-atpF_p2	atpA-atpF	2	ACTTGAAGTACGGTACCGGT	TGGTATGTTTGGAGCGATGA	20	20	50.0	45.0	58.4	56.9	35.9	0.0	1.449000	12817	12880	204-205	1.000000	68.8
atpA-atpF_p3	atpA-atpF	3	TTGCTCAATACGTTCACGGA	TGGTATGTTTGGAGCGATGA	20	20	45.0	45.0	57.6	56.9	4.1	0.0	1.512035	12817	12880	157-158	1.000000	68.4
atpA-atpF_p4	atpA-atpF	4	TCGCCTACTTGAAGTACGGT	TGGTATGTTTGGAGCGATGA	20	20	50.0	45.0	58.5	56.9	13.2	0.0	1.531775	12817	12880	210-211	1.000000	68.1
atpA-atpF_p5	atpA-atpF	5	GAATTTCGTCGGCTCGAATG	TGGTATGTTTGGAGCGATGAA	20	21	50.0	42.9	57.6	57.7	13.2	0.0	1.695634	12817	12880	128-129	1.000000	67.1
atpF_p1	atpF	1	GGCTCGAATGGTTACCATGA	AAAAAGGGGCGAAGTGATAA	20	20	50.0	40.0	57.7	55.2	18.5	0.0	3.092367	12881	14157	1426-1427	1.000000	55.3
atpF_p2	atpF	2	GGCTCGAATGGTTACCATGA	AAAAGGGGCGAAGTGATAAA	20	20	50.0	40.0	57.7	55.2	18.5	0.0	3.092367	12881	14157	1425-1426	1.000000	55.3
atpF_p3	atpF	3	GGCTCGAATGGTTACCATGA	AAAGGGGCGAAGTGATAAAA	20	20	50.0	40.0	57.7	55.2	18.5	0.0	3.092367	12881	14157	1424-1425	1.000000	55.3
atpF_p4	atpF	4	GGCTCGAATGGTTACCATGA	AAGGGGCGAAGTGATAAAAA	20	20	50.0	40.0	57.7	55.2	18.5	0.0	3.092367	12881	14157	1423-1424	1.000000	55.3
atpF_p5	atpF	5	GAATTTCGTCGGCTCGAATG	AAGGGGCGAAGTGATAAAAA	20	20	50.0	40.0	57.6	55.2	13.2	0.0	3.120041	12881	14157	1433-1434	1.000000	55.1