Hotspot

ycf1

SSC · 131658-137435 · 5778 bp

Back to Pterospermum

Pi 0.0122

MarkerSeek score 33.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5778
variable_sites: 105
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTGCTCCTGTTTATCCCCC	GATCTACTATGCGCCTCTGC	55.0	55.0	57.9	58.0	689-692 bp	1.0	83.5
ycf1_p2	CACTTTCCCCCACTTCTTCC	GATCTACTATGCGCCTCTGC	55.0	55.0	58.1	58.0	626-629 bp	1.0	83.5
ycf1_p3	CTTCGGTGGATACCTCTTGC	GATCTACTATGCGCCTCTGC	55.0	55.0	58.1	58.0	704-707 bp	1.0	83.4
ycf1_p4	GAAAGTGGGGTTCGTCCTTT	GATCTACTATGCGCCTCTGC	50.0	55.0	58.0	58.0	821-824 bp	1.0	83.2
ycf1_p5	GAAAGTGGGGTTCGTCCTTT	TTCGGTCGTTATGGTTGGAC	50.0	50.0	58.0	57.9	992-995 bp	1.0	82.1