Hotspot

ndhF-rpl32

SSC · 119191-120469 · 1279 bp

Back to Pterospermum

Pi 0.162

MarkerSeek score 83.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1279
variable_sites: 272
pi: 0.162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	TTTCAACGCCGCCCAATATA	45.0	45.0	58.0	58.0	757-1468 bp	1.0	91.3
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	AACGCCGCCCAATATACTTT	45.0	45.0	58.0	58.0	753-1464 bp	1.0	91.2
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	CGCCGCCCAATATACTTTCT	45.0	50.0	58.0	58.1	751-1462 bp	1.0	90.6
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	TGAAAAAGCTTTCAACGCCG	45.0	45.0	58.0	58.2	766-1477 bp	1.0	90.3
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTTCAACGCCGCCCAATATA	45.0	45.0	57.8	58.0	759-1470 bp	1.0	90.1