Hotspot

rpl22-rps19

LSC · 90822-91078 · 257 bp

Back to Pterospermum

Pi 0.0419

MarkerSeek score 60.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 11
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AGTTGGATATGGGTTCCTCT	TTGGGAGAATTCGCACCTAC	45.0	50.0	55.2	57.6	286-368 bp	1.0	61.1
rpl22-rps19_p2	TCGTTTGTAGTTGGATATGGGT	TTGGGAGAATTCGCACCTAC	40.9	50.0	57.2	57.6	294-376 bp	1.0	60.9
rpl22-rps19_p3	AGTTGGATATGGGTTCCTCT	TGGGAGAATTCGCACCTACT	45.0	50.0	55.2	58.4	285-367 bp	1.0	60.8
rpl22-rps19_p4	TCGTTTGTAGTTGGATATGGGT	TGGGAGAATTCGCACCTACT	40.9	50.0	57.2	58.4	293-375 bp	1.0	60.7
rpl22-rps19_p5	AGTTGGATATGGGTTCCTCT	AATTGGGAGAATTCGCACCT	45.0	45.0	55.2	57.5	288-370 bp	1.0	60.1