Hotspot

atpB-rbcL

LSC · 59679-60680 · 1002 bp

Back to Pterospermum

Pi 0.0115

MarkerSeek score 52.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 17
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.0	58.1	1080-1163 bp	1.0	80.9
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.0	58.4	1081-1164 bp	1.0	78.5
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	45.0	55.0	58.0	57.3	1082-1165 bp	1.0	76.3
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	AAGTCCCTCCCTACAACTCA	45.0	50.0	58.0	57.3	1072-1155 bp	1.0	76.1
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	AGTCCCTCCCTACAACTCAT	45.0	50.0	58.0	57.0	1071-1154 bp	1.0	74.2