Hotspot

atpF-atpH

LSC · 14159-14820 · 662 bp

Back to Pterospermum

Pi 0.0187

MarkerSeek score 71.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 18
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	747-785 bp	1.0	83.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.9	58.0	775-813 bp	1.0	83.0
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	50.0	50.0	58.0	58.5	748-786 bp	1.0	80.1
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	50.0	50.0	58.0	58.5	753-791 bp	1.0	79.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	50.0	50.0	57.9	58.5	776-814 bp	1.0	79.4