Hotspot

trnK-UUU-rps16

LSC · 4980-5920 · 941 bp

Back to Pterospermum

Pi 0.00607

MarkerSeek score 54.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 8
pi: 0.00607
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1032-1069 bp	1.0	83.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1031-1068 bp	1.0	83.2
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	1018-1055 bp	1.0	80.8
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	992-1029 bp	1.0	80.3
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	991-1028 bp	1.0	80.3