Hotspot

trnG-GCC

LSC · 25544-25614 · 71 bp

Back to Pterodon

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	CGTCTCCGCAACAGTTTTTG	GACTGACCTAGCTTTGCACA	50.0	50.0	58.3	57.8	238 bp	1.0	76.9
trnG-GCC_p2	AGAAGAAGAAGAGGGGAGGG	GACTGACCTAGCTTTGCACA	55.0	50.0	57.7	57.8	201 bp	1.0	76.9
trnG-GCC_p3	AGAAGAGGGGAGGGGGATAT	GACTGACCTAGCTTTGCACA	55.0	50.0	58.4	57.8	195 bp	1.0	75.7
trnG-GCC_p4	GAAGAGGGGAGGGGGATATT	GACTGACCTAGCTTTGCACA	55.0	50.0	57.6	57.8	194 bp	1.0	75.5
trnG-GCC_p5	CGTCTCCGCAACAGTTTTTG	AGACTGACCTAGCTTTGCAC	50.0	50.0	58.3	57.5	239 bp	1.0	74.6