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atpA

LSC · 52857-54371 · 1515 bp

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Pi 0.00198

MarkerSeek score 59.90

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGGCATGTTTGAGGCAATGA	TTGAACAAGAGCCACTTAGG	45.0	45.0	58.4	55.3	1703 bp	1.0	55.5
atpA_p2	TTGGCATGTTTGAGGCAATG	TTGAACAAGAGCCACTTAGG	45.0	45.0	57.5	55.3	1704 bp	1.0	54.7
atpA_p3	TGGCATGTTTGAGGCAATGA	TGAATTGAACAAGAGCCACT	45.0	40.0	58.4	55.2	1707 bp	1.0	54.6
atpA_p4	TTGGCATGTTTGAGGCAATG	TGAATTGAACAAGAGCCACT	45.0	40.0	57.5	55.2	1708 bp	1.0	53.8
atpA_p5	TGGCATGTTTGAGGCAATGA	TGAATTGAACAAGAGCCACTT	45.0	38.1	58.4	56.0	1707 bp	1.0	53.1