Hotspot

ndhF-trnN-GUU

IRa · 134131-135079 · 949 bp

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Pi 0.00105

MarkerSeek score 59.40

Primer available yes

Label ndhF-trnN-GUU

Feature Metadata

label_name: ndhF-trnN-GUU
feature_type: igs
region: IRa
length_bp: 949
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN-GUU_p1	GGGGTGGACGAATTTCTTCA	ACGACCAGTCAGTTAACAGC	50.0	50.0	57.8	57.8	1087 bp	1.0	77.3
ndhF-trnN-GUU_p2	GGGGTGGACGAATTTCTTCA	AGGATTCGAACCTACGACCA	50.0	50.0	57.8	58.2	1100 bp	1.0	77.3
ndhF-trnN-GUU_p3	GGGGTGGACGAATTTCTTCA	GGATTCGAACCTACGACCAG	50.0	55.0	57.8	57.8	1099 bp	1.0	76.9
ndhF-trnN-GUU_p4	GGGGTGGACGAATTTCTTCA	CCCCAAGTAGGATTCGAACC	50.0	55.0	57.8	57.7	1108 bp	1.0	75.9
ndhF-trnN-GUU_p5	GGGGTGGACGAATTTCTTCA	TTCGAACCTACGACCAGTCA	50.0	50.0	57.8	58.4	1096 bp	1.0	75.4