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rpl32-ndhF

SSC · 130846-131883 · 1038 bp

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Pi 0.00193

MarkerSeek score 60.10

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1038
variable_sites: 2
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCAATGCTGCCCAATATCCC	TGTTGCCGTGGGAAAAAGTA	50.0	45.0	58.0	57.9	1223-1225 bp	1.0	79.9
rpl32-ndhF_p2	CAATGCTGCCCAATATCCCT	TGTTGCCGTGGGAAAAAGTA	50.0	45.0	58.0	57.9	1222-1224 bp	1.0	79.9
rpl32-ndhF_p3	AAAAGCTTTCAATGCTGCCC	TGTTGCCGTGGGAAAAAGTA	45.0	45.0	57.8	57.9	1231-1233 bp	1.0	78.5
rpl32-ndhF_p4	TCAATGCTGCCCAATATCCC	TTTGTTGCCGTGGGAAAAAG	50.0	45.0	58.0	57.7	1225-1227 bp	1.0	77.9
rpl32-ndhF_p5	CAATGCTGCCCAATATCCCT	TTTGTTGCCGTGGGAAAAAG	50.0	45.0	58.0	57.7	1224-1226 bp	1.0	77.9