Hotspot

rps11-rpl36

LSC · 83744-84296 · 553 bp

Back to Pterodon

Pi 0.00723

MarkerSeek score 61.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 4
pi: 0.00723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ATTTTACGCGCATGCTTACG	AGACGGGGTCGAATTAGAGT	45.0	50.0	57.9	57.9	687-691 bp	1.0	78.7
rps11-rpl36_p2	TTGGTATTTTACGCGCATGC	AGACGGGGTCGAATTAGAGT	45.0	50.0	57.8	57.9	692-696 bp	1.0	77.9
rps11-rpl36_p3	CCCTTTGGTATTTTACGCGC	AGACGGGGTCGAATTAGAGT	50.0	50.0	57.8	57.9	696-700 bp	1.0	77.9
rps11-rpl36_p4	ATTTTACGCGCATGCTTACG	CGTAGACGGGGTCGAATTAG	45.0	55.0	57.9	57.6	690-694 bp	1.0	76.6
rps11-rpl36_p5	TTACGCGAACCAATACGTCC	AGACGGGGTCGAATTAGAGT	50.0	50.0	58.4	57.9	672-676 bp	1.0	76.4