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trnQ-UUG-accD

LSC · 58279-60632 · 2354 bp

Back to Pterodon

Pi 0.023

MarkerSeek score 70.60

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: LSC
length_bp: 2354
variable_sites: 54
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GTCCCGGTATTCGAAGGTTC	TGGGCCTTCACTTACATTGG	55.0	50.0	58.1	57.8	2507-2511 bp	1.0	79.7
trnQ-UUG-accD_p2	GTCCCGGTATTCGAAGGTTC	GGGCCTTCACTTACATTGGT	55.0	50.0	58.1	57.8	2506-2510 bp	1.0	79.7
trnQ-UUG-accD_p3	GTTTTGGTCCCGGTATTCGA	TGGGCCTTCACTTACATTGG	50.0	50.0	57.9	57.8	2513-2517 bp	1.0	79.5
trnQ-UUG-accD_p4	GTTTTGGTCCCGGTATTCGA	GGGCCTTCACTTACATTGGT	50.0	50.0	57.9	57.8	2512-2516 bp	1.0	79.5
trnQ-UUG-accD_p5	AAGGTTCGAATCCTTCCGTC	TGGGCCTTCACTTACATTGG	50.0	50.0	57.6	57.8	2494-2498 bp	1.0	77.3