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psbK-trnQ-UUG

LSC · 57393-58206 · 814 bp

Back to Pterodon

Pi 0.00616

MarkerSeek score 62.50

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 5
pi: 0.00616
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGGCTTCTGGTAATTTCGCC	GAACCTTCGAATACCGGGAC	50.0	55.0	58.2	58.1	955-957 bp	1.0	78.1
psbK-trnQ-UUG_p2	AGGCTTCTGGTAATTTCGCC	TCGAATACCGGGACCAAAAC	50.0	50.0	58.2	57.9	949-951 bp	1.0	77.8
psbK-trnQ-UUG_p3	AGGCTTCTGGTAATTTCGCC	AATGCATATGCTCTGGGACG	50.0	50.0	58.2	58.1	984-986 bp	1.0	77.7
psbK-trnQ-UUG_p4	AGGCTTCTGGTAATTTCGCC	CATATGCTCTGGGACGGAAG	50.0	55.0	58.2	57.8	980-982 bp	1.0	77.3
psbK-trnQ-UUG_p5	GGCTTCTGGTAATTTCGCCA	GAACCTTCGAATACCGGGAC	50.0	55.0	58.5	58.1	954-956 bp	1.0	75.7