Hotspot

trnR-UCU-trnG-UCC

LSC · 54579-54876 · 298 bp

Back to Pterodon

Pi 0.0302

MarkerSeek score 67.60

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 9
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AGAAGACCTCTGTCCTATCCA	ACCCTTAGCCTTCCAAGCTA	47.6	50.0	56.9	58.0	384 bp	1.0	65.2
trnR-UCU-trnG-UCC_p2	AGGTTTAGAAGACCTCTGTCCT	ACCCTTAGCCTTCCAAGCTA	45.5	50.0	57.9	58.0	390 bp	1.0	64.5
trnR-UCU-trnG-UCC_p3	AGAAGACCTCTGTCCTATCCA	ACCAAAGTTTCGTGTTCGGT	47.6	45.0	56.9	58.3	448 bp	1.0	63.1
trnR-UCU-trnG-UCC_p4	AGGTTTAGAAGACCTCTGTCCT	ACCAAAGTTTCGTGTTCGGT	45.5	45.0	57.9	58.3	454 bp	1.0	62.4
trnR-UCU-trnG-UCC_p5	AGAAGACCTCTGTCCTATCCAT	ACCCTTAGCCTTCCAAGCTA	45.5	50.0	57.4	58.0	384 bp	1.0	61.2