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ycf3-psaA

LSC · 19270-20298 · 1029 bp

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Pi 0.0155

MarkerSeek score 67.70

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 16
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TTGGTTGAAGATCACAGGGC	TGAGAAATGACCGGGTTTGG	50.0	50.0	58.1	58.1	1159-1165 bp	1.0	79.6
ycf3-psaA_p2	TTGGTTGAAGATCACAGGGC	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.1	57.9	1140-1146 bp	1.0	79.5
ycf3-psaA_p3	AGATCACAGGGCGTTTTGAG	TGAGAAATGACCGGGTTTGG	50.0	50.0	58.2	58.1	1151-1157 bp	1.0	78.9
ycf3-psaA_p4	AGATCACAGGGCGTTTTGAG	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.2	57.9	1132-1138 bp	1.0	78.7
ycf3-psaA_p5	AAGCTATAGCCCTTACCCCC	TGAGAAATGACCGGGTTTGG	55.0	50.0	58.3	58.1	1202-1208 bp	1.0	78.2