primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.2	58.0	20.3	13.4	0.203950	4301	5550	1359-1359	1.000000	79.1
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.2	58.0	20.3	13.4	0.203950	4301	5550	1358-1358	1.000000	79.1
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AAAGCCGAGTACTCTACCGT	GAGGCTAGCAAATGGAGTCC	20	20	50.0	55.0	58.2	58.0	20.3	18.2	0.208003	4301	5550	1374-1374	1.000000	79.1
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	AAGCCGAGTACTCTACCGTT	GAGGCTAGCAAATGGAGTCC	20	20	50.0	55.0	58.2	58.0	20.3	18.2	0.208003	4301	5550	1373-1373	1.000000	79.1
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	AAGCCGAGTACTCTACCGTT	GGCTAGCAAATGGAGTCCTG	20	20	50.0	55.0	58.2	58.3	20.3	18.2	0.495251	4301	5550	1371-1371	1.000000	76.8
ycf3-psaA_p1	ycf3-psaA	1	TTGGTTGAAGATCACAGGGC	TGAGAAATGACCGGGTTTGG	20	20	50.0	50.0	58.1	58.1	0.0	6.3	0.186013	19270	20298	1159-1165	1.000000	79.6
ycf3-psaA_p2	ycf3-psaA	2	TTGGTTGAAGATCACAGGGC	GCCCATTCCTCGAAAGAAGT	20	20	50.0	50.0	58.1	57.9	0.0	4.1	0.205643	19270	20298	1140-1146	1.000000	79.5
ycf3-psaA_p3	ycf3-psaA	3	AGATCACAGGGCGTTTTGAG	TGAGAAATGACCGGGTTTGG	20	20	50.0	50.0	58.2	58.1	0.0	6.3	0.282200	19270	20298	1151-1157	1.000000	78.9
ycf3-psaA_p4	ycf3-psaA	4	AGATCACAGGGCGTTTTGAG	GCCCATTCCTCGAAAGAAGT	20	20	50.0	50.0	58.2	57.9	0.0	4.1	0.301829	19270	20298	1132-1138	1.000000	78.7
ycf3-psaA_p5	ycf3-psaA	5	AAGCTATAGCCCTTACCCCC	TGAGAAATGACCGGGTTTGG	20	20	55.0	50.0	58.3	58.1	21.2	6.3	0.352124	19270	20298	1202-1208	1.000000	78.2
trnG-GCC_p1	trnG-GCC	1	CGTCTCCGCAACAGTTTTTG	GACTGACCTAGCTTTGCACA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.427840	25544	25614	238-238	1.000000	76.9
trnG-GCC_p2	trnG-GCC	2	AGAAGAAGAAGAGGGGAGGG	GACTGACCTAGCTTTGCACA	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.432098	25544	25614	201-201	1.000000	76.9
trnG-GCC_p3	trnG-GCC	3	AGAAGAGGGGAGGGGGATAT	GACTGACCTAGCTTTGCACA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.594224	25544	25614	195-195	1.000000	75.7
trnG-GCC_p4	trnG-GCC	4	GAAGAGGGGAGGGGGATATT	GACTGACCTAGCTTTGCACA	20	20	55.0	50.0	57.6	57.8	0.0	0.0	0.612094	25544	25614	194-194	1.000000	75.5
trnG-GCC_p5	trnG-GCC	5	CGTCTCCGCAACAGTTTTTG	AGACTGACCTAGCTTTGCAC	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.722164	25544	25614	239-239	1.000000	74.6
atpI-atpH_p1	atpI-atpH	1	GCTGCGGCTTATATAGGTGA	GATCCCTTCTACAGCTTGCC	20	20	50.0	55.0	57.3	58.0	11.2	0.0	0.783219	49226	50592	1526-1526	1.000000	73.9
atpI-atpH_p2	atpI-atpH	2	ACAAGCGGTATTCAAGCTCT	GATCCCTTCTACAGCTTGCC	20	20	45.0	55.0	57.2	58.0	0.0	0.0	0.809747	49226	50592	1562-1562	1.000000	73.7
atpI-atpH_p3	atpI-atpH	3	TAGGTGAGTCCATGGAAGGC	GATCCCTTCTACAGCTTGCC	20	20	55.0	55.0	58.8	58.0	18.2	0.0	0.835438	49226	50592	1513-1513	1.000000	73.5
atpI-atpH_p4	atpI-atpH	4	GCTGCGGCTTATATAGGTGA	AACGGAAGCAGCAGAAATCA	20	20	50.0	45.0	57.3	58.1	11.2	0.0	0.847416	49226	50592	1439-1439	1.000000	73.4
atpI-atpH_p5	atpI-atpH	5	GCTGCGGCTTATATAGGTGA	GTCCAATAGAAGCAAGCCCA	20	20	50.0	50.0	57.3	57.9	11.2	0.0	0.872406	49226	50592	1479-1479	1.000000	73.2
atpA_p1	atpA	1	TGGCATGTTTGAGGCAATGA	TTGAACAAGAGCCACTTAGG	20	20	45.0	45.0	58.4	55.3	0.0	0.0	3.085254	52857	54371	1703-1703	1.000000	55.5
atpA_p2	atpA	2	TTGGCATGTTTGAGGCAATG	TTGAACAAGAGCCACTTAGG	20	20	45.0	45.0	57.5	55.3	0.0	0.0	3.174537	52857	54371	1704-1704	1.000000	54.7
atpA_p3	atpA	3	TGGCATGTTTGAGGCAATGA	TGAATTGAACAAGAGCCACT	20	20	45.0	40.0	58.4	55.2	0.0	0.0	3.198356	52857	54371	1707-1707	1.000000	54.6
atpA_p4	atpA	4	TTGGCATGTTTGAGGCAATG	TGAATTGAACAAGAGCCACT	20	20	45.0	40.0	57.5	55.2	0.0	0.0	3.287640	52857	54371	1708-1708	1.000000	53.8
atpA_p5	atpA	5	TGGCATGTTTGAGGCAATGA	TGAATTGAACAAGAGCCACTT	20	21	45.0	38.1	58.4	56.0	0.0	0.0	3.383886	52857	54371	1707-1707	1.000000	53.1
trnR-UCU-trnG-UCC_p1	trnR-UCU-trnG-UCC	1	AGAAGACCTCTGTCCTATCCA	ACCCTTAGCCTTCCAAGCTA	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.084759	54579	54876	384-384	1.000000	65.2
trnR-UCU-trnG-UCC_p2	trnR-UCU-trnG-UCC	2	AGGTTTAGAAGACCTCTGTCCT	ACCCTTAGCCTTCCAAGCTA	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.168087	54579	54876	390-390	1.000000	64.5
trnR-UCU-trnG-UCC_p3	trnR-UCU-trnG-UCC	3	AGAAGACCTCTGTCCTATCCA	ACCAAAGTTTCGTGTTCGGT	21	20	47.6	45.0	56.9	58.3	0.0	0.0	2.310076	54579	54876	448-448	1.000000	63.1
trnR-UCU-trnG-UCC_p4	trnR-UCU-trnG-UCC	4	AGGTTTAGAAGACCTCTGTCCT	ACCAAAGTTTCGTGTTCGGT	22	20	45.5	45.0	57.9	58.3	0.0	0.0	2.393404	54579	54876	454-454	1.000000	62.4
trnR-UCU-trnG-UCC_p5	trnR-UCU-trnG-UCC	5	AGAAGACCTCTGTCCTATCCAT	ACCCTTAGCCTTCCAAGCTA	22	20	45.5	50.0	57.4	58.0	0.0	0.0	2.584370	54579	54876	384-384	1.000000	61.2
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	AGGCTTCTGGTAATTTCGCC	GAACCTTCGAATACCGGGAC	20	20	50.0	55.0	58.2	58.1	0.0	6.3	0.316657	57393	58206	955-957	1.000000	78.1
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	AGGCTTCTGGTAATTTCGCC	TCGAATACCGGGACCAAAAC	20	20	50.0	50.0	58.2	57.9	0.0	6.3	0.346243	57393	58206	949-951	1.000000	77.8
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	AGGCTTCTGGTAATTTCGCC	AATGCATATGCTCTGGGACG	20	20	50.0	50.0	58.2	58.1	0.0	27.1	0.360348	57393	58206	984-986	1.000000	77.7
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	AGGCTTCTGGTAATTTCGCC	CATATGCTCTGGGACGGAAG	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.409385	57393	58206	980-982	1.000000	77.3
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	GGCTTCTGGTAATTTCGCCA	GAACCTTCGAATACCGGGAC	20	20	50.0	55.0	58.5	58.1	0.0	6.3	0.608644	57393	58206	954-956	1.000000	75.7
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GTCCCGGTATTCGAAGGTTC	TGGGCCTTCACTTACATTGG	20	20	55.0	50.0	58.1	57.8	3.3	4.5	0.282470	58279	60632	2507-2511	1.000000	79.7
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GTCCCGGTATTCGAAGGTTC	GGGCCTTCACTTACATTGGT	20	20	55.0	50.0	58.1	57.8	3.3	0.8	0.284036	58279	60632	2506-2510	1.000000	79.7
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GTTTTGGTCCCGGTATTCGA	TGGGCCTTCACTTACATTGG	20	20	50.0	50.0	57.9	57.8	0.0	4.5	0.312056	58279	60632	2513-2517	1.000000	79.5
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GTTTTGGTCCCGGTATTCGA	GGGCCTTCACTTACATTGGT	20	20	50.0	50.0	57.9	57.8	0.0	0.8	0.313622	58279	60632	2512-2516	1.000000	79.5
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	AAGGTTCGAATCCTTCCGTC	TGGGCCTTCACTTACATTGG	20	20	50.0	50.0	57.6	57.8	19.8	4.5	0.596101	58279	60632	2494-2498	1.000000	77.3
accD-psaI_p1	accD-psaI	1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	4.9	26.9	0.027955	62115	62679	727-730	1.000000	80.3
accD-psaI_p2	accD-psaI	2	GCGTAGTCCTTTAAAGGGGG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	29.7	26.9	0.061108	62115	62679	711-714	1.000000	80.1
accD-psaI_p3	accD-psaI	3	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	4.9	26.8	0.104489	62115	62679	733-736	1.000000	79.7
accD-psaI_p4	accD-psaI	4	GCGTAGTCCTTTAAAGGGGG	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.0	57.9	29.7	26.8	0.137641	62115	62679	717-720	1.000000	79.5
accD-psaI_p5	accD-psaI	5	TTCAATCGTACCGCGTAGTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	4.9	26.9	0.205477	62115	62679	723-726	1.000000	78.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.269767	70231	71083	972-972	1.000000	78.0
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.271110	70231	71083	971-971	1.000000	78.0
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.277803	70231	71083	983-983	1.000000	77.9
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	70231	71083	982-982	1.000000	77.9
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	AAGCCCCGAACCATAGAGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.279136	70231	71083	985-985	1.000000	77.9
rps11-rpl36_p1	rps11-rpl36	1	ATTTTACGCGCATGCTTACG	AGACGGGGTCGAATTAGAGT	20	20	45.0	50.0	57.9	57.9	17.1	0.0	0.226371	83744	84296	687-691	1.000000	78.7
rps11-rpl36_p2	rps11-rpl36	2	TTGGTATTTTACGCGCATGC	AGACGGGGTCGAATTAGAGT	20	20	45.0	50.0	57.8	57.9	5.7	0.0	0.322264	83744	84296	692-696	1.000000	77.9
rps11-rpl36_p3	rps11-rpl36	3	CCCTTTGGTATTTTACGCGC	AGACGGGGTCGAATTAGAGT	20	20	50.0	50.0	57.8	57.9	5.7	0.0	0.323743	83744	84296	696-700	1.000000	77.9
rps11-rpl36_p4	rps11-rpl36	4	ATTTTACGCGCATGCTTACG	CGTAGACGGGGTCGAATTAG	20	20	45.0	55.0	57.9	57.6	17.1	0.0	0.481692	83744	84296	690-694	1.000000	76.6
rps11-rpl36_p5	rps11-rpl36	5	TTACGCGAACCAATACGTCC	AGACGGGGTCGAATTAGAGT	20	20	50.0	50.0	58.4	57.9	15.9	0.0	0.512960	83744	84296	672-676	1.000000	76.4
rps8_p1	rps8	1	ACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAA	20	20	40.0	40.0	54.0	54.6	0.0	0.0	7.410011	84857	85267	564-564	1.000000	40.0
rps8_p2	rps8	2	AACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAA	21	20	38.1	40.0	54.9	54.6	0.0	0.0	7.543636	84857	85267	565-565	1.000000	40.0
rps8_p3	rps8	3	ACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAAA	20	21	40.0	38.1	54.0	55.4	0.0	0.0	7.572905	84857	85267	564-564	1.000000	40.0
rps8_p4	rps8	4	ACTTCCTTCACGTATTCACT	ATCGAAAGTCAGAACCAAGA	20	20	40.0	40.0	54.0	54.3	0.0	0.0	7.648823	84857	85267	565-565	1.000000	40.0
rps8_p5	rps8	5	AACTTCCTTCACGTATTCACT	TCGAAAGTCAGAACCAAGAAA	21	21	38.1	38.1	54.9	55.4	0.0	0.0	7.706531	84857	85267	565-565	1.000000	40.0
rps8-rpl14_p1	rps8-rpl14	1	ATTACGTCAGCGATGGTGTC	AATTTTTGGTGCAATCGCCC	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.270291	85268	85526	367-367	1.000000	80.5
rps8-rpl14_p2	rps8-rpl14	2	GTCAGCGATGGTGTCCTTAC	AATTTTTGGTGCAATCGCCC	20	20	55.0	45.0	58.4	58.2	0.0	0.0	0.551284	85268	85526	362-362	1.000000	78.2
rps8-rpl14_p3	rps8-rpl14	3	ATTACGTCAGCGATGGTGTC	CGAATTTTTGGTGCAATCGC	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.844995	85268	85526	369-369	1.000000	75.8
rps8-rpl14_p4	rps8-rpl14	4	ATTACGTCAGCGATGGTGTC	GCCCGGGAATTGAAACAGTT	20	20	50.0	50.0	58.1	59.0	0.0	29.3	1.111250	85268	85526	351-351	1.000000	73.8
rps8-rpl14_p5	rps8-rpl14	5	GTCAGCGATGGTGTCCTTAC	CGAATTTTTGGTGCAATCGC	20	20	55.0	45.0	58.4	57.2	0.0	0.0	1.125989	85268	85526	364-364	1.000000	73.6
rps12_p1	rps12	1	CAGTACCTCGACGTGACATG	TACGGTTCTGTAGAGTGGCA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.189877	102571	103489	1038-1038	1.000000	78.6
rps12_p2	rps12	2	GTACCTCGACGTGACATGAG	TACGGTTCTGTAGAGTGGCA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.261752	102571	103489	1036-1036	1.000000	78.0
rps12_p3	rps12	3	CAGTACCTCGACGTGACATG	GAACGAGGAGCCGTATGAAG	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.262205	102571	103489	1071-1071	1.000000	78.0
rps12_p4	rps12	4	AGTACCTCGACGTGACATGA	TACGGTTCTGTAGAGTGGCA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.268865	102571	103489	1037-1037	1.000000	77.9
rps12_p5	rps12	5	CAGTACCTCGACGTGACATG	ATTGAACGAGGAGCCGTATG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.328723	102571	103489	1074-1074	1.000000	77.4
rpl32-ndhF_p1	rpl32-ndhF	1	TCAATGCTGCCCAATATCCC	TGTTGCCGTGGGAAAAAGTA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.054584	130846	131883	1223-1225	1.000000	79.9
rpl32-ndhF_p2	rpl32-ndhF	2	CAATGCTGCCCAATATCCCT	TGTTGCCGTGGGAAAAAGTA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.059602	130846	131883	1222-1224	1.000000	79.9
rpl32-ndhF_p3	rpl32-ndhF	3	AAAAGCTTTCAATGCTGCCC	TGTTGCCGTGGGAAAAAGTA	20	20	45.0	45.0	57.8	57.9	13.1	0.0	0.228596	130846	131883	1231-1233	1.000000	78.5
rpl32-ndhF_p4	rpl32-ndhF	4	TCAATGCTGCCCAATATCCC	TTTGTTGCCGTGGGAAAAAG	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.301929	130846	131883	1225-1227	1.000000	77.9
rpl32-ndhF_p5	rpl32-ndhF	5	CAATGCTGCCCAATATCCCT	TTTGTTGCCGTGGGAAAAAG	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.306947	130846	131883	1224-1226	1.000000	77.9
ndhF-trnN-GUU_p1	ndhF-trnN-GUU	1	GGGGTGGACGAATTTCTTCA	ACGACCAGTCAGTTAACAGC	20	20	50.0	50.0	57.8	57.8	5.4	8.6	0.344599	134131	135079	1087-1087	1.000000	77.3
ndhF-trnN-GUU_p2	ndhF-trnN-GUU	2	GGGGTGGACGAATTTCTTCA	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.8	58.2	5.4	15.0	0.350833	134131	135079	1100-1100	1.000000	77.3
ndhF-trnN-GUU_p3	ndhF-trnN-GUU	3	GGGGTGGACGAATTTCTTCA	GGATTCGAACCTACGACCAG	20	20	50.0	55.0	57.8	57.8	5.4	0.0	0.399508	134131	135079	1099-1099	1.000000	76.9
ndhF-trnN-GUU_p4	ndhF-trnN-GUU	4	GGGGTGGACGAATTTCTTCA	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	57.8	57.7	5.4	0.0	0.521401	134131	135079	1108-1108	1.000000	75.9
ndhF-trnN-GUU_p5	ndhF-trnN-GUU	5	GGGGTGGACGAATTTCTTCA	TTCGAACCTACGACCAGTCA	20	20	50.0	50.0	57.8	58.4	5.4	0.0	0.586147	134131	135079	1096-1096	1.000000	75.4