Hotspot

ycf1

SSC · 127391-132865 · 5475 bp

Back to Pterocyclus

Pi 0.00676

MarkerSeek score 44.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5475
variable_sites: 72
pi: 0.00676
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGTCATACCACCTTCAGT	TCCGGCGAAACTAACCAAAA	50.0	45.0	58.0	58.0	872 bp	0.75	71.1
ycf1_p2	TGGGTCATACCACCTTCAGT	AATCCGGCGAAACTAACCAA	50.0	45.0	58.0	57.8	874 bp	0.75	69.9
ycf1_p3	TGGGTCATACCACCTTCAGT	ATCCGGCGAAACTAACCAAA	50.0	45.0	58.0	57.8	873 bp	0.75	69.9
ycf1_p4	TGGTCCAGGCCTCAATATCT	TCCGGCGAAACTAACCAAAA	50.0	45.0	57.8	58.0	1276-1288 bp	0.75	69.7
ycf1_p5	TGGGTCATACCACCTTCAGT	GAATCCGGCGAAACTAACCA	50.0	50.0	58.0	58.3	875 bp	0.75	69.3