Hotspot

rpoC1

LSC · 22008-24810 · 2803 bp

Back to Pterocyclus

Pi 0.00488

MarkerSeek score 46.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2803
variable_sites: 25
pi: 0.00488
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGAATTACTTAGCTTACGGACCA	TTGCTCGTTCGAGAACTACG	39.1	50.0	57.1	58.0	2946-2950 bp	1.0	49.2
rpoC1_p2	AGAATTACTTAGCTTACGGACCA	ACGATCTTTGGCTCTGGAAC	39.1	50.0	57.1	57.9	2929-2933 bp	1.0	48.7
rpoC1_p3	AGAATTACTTAGCTTACGGACCA	AGGAGGGAAGCTTAATCGGA	39.1	50.0	57.1	57.8	2868-2872 bp	1.0	48.1
rpoC1_p4	AGAATTACTTAGCTTACGGACCA	CGATCTTTGGCTCTGGAACT	39.1	50.0	57.1	57.6	2928-2932 bp	1.0	46.3
rpoC1_p5	AGAATTACTTAGCTTACGGACCA	GCTCGTTCGAGAACTACGAT	39.1	50.0	57.1	57.5	2944-2948 bp	1.0	45.8